Protective mechanism on gastric lesion of Sennoside A and Sennoside B

Abstract

Sennoside A and sennoside B is evacuant to increase the sensitivity of the colon. In vivo test, we performed HCl·ethanol‐induced gastritis test, indomethacin‐induced gastric ulcer test, gastric secretion in pylorus‐ligated test, H+/K+ATPase activity test, gastric emptying and intestinal motility test. In vitro test, we examined acid‐neutralizing capacity, quantity of PGE2 and inhibition of H. pylori colonization and we confirmed apoptosis using DAPI nuclear staining, FACS analysis. Sennoside A and B inhibit lesion index in gastritis and gastric ulcer in rats. These results showed that these components reduced gastric juice, an aggressive factor and total acidity and increased pH moderately. In addition, it was made sure that proton pump inhibiton influenced on gastric acid secretion control and that colonization inhibiting activity on H. pylori. As protection enhancing factors to gastric damage, sennoside A and B increased PGE2 in a concentration‐dependent manner. Especially, sennoside B was superior to sennoside A in proton pump inhibitory activity and PGE2 synthesis increase. From gastric emptying rate experiment, both sennoside A and B showed the recovery of stomach adaptability and gastric movement acceleration and that of motility of duodeno‐jejunum disorder from intestinal transporting rate experiment compared to control group. To make use of DAPI nuclear stain method, nuclear change has been identified and the rate of apoptosis increase confirmed concentration dependently with FACS. These results are expected to contribute the standardization and scientific movement of herbal medicines and furtherly to make sure of the capability of development of herbal drugs.