Protective mechanism of the resveratrol in liver injury of obstructive jaundice in rats

Abstract

Objective To explore the protective mechanism and effect of the resveratrol (Res) for liver injury of obstructive jaundice.Methods The rats were divided randomly into three groups:the sham group receiving laparotomy without bile duct ligation (BDL),the BDL group and the BDL + Res group with the Res given following BDL.The expression of the silent information regulator 1 (SIRT1) and nuclear factor-κBp56 (NF-κB) proteins were analyzed by western blotting but immunocytochemical assay was performed to examine peroxisome proliferator activated receptor-alpha (PPARα) protein.Real-time polymerase chain reaction (PCR) was performed to determine the mRNA expression of SIRT1 and PPARα.Cell apoptosis was examined by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining.Results After the BDL,the cholestasis model was established.Compared B group to A group,the alanine transaminase (ALT) was higher and the mRNA and proteins expression of the SIRT1 and the PPARα was lower,but the NF-κB protein and the rate of cell apoptosis was higher (P ＜ 0.05).Compared B group to C group,the ALT was higher and the mRNA and proteins expression of the SIRT1 and the PPARα was lower,but the NF-κB protein and the rate of cell apoptosis was significantly higher (P ＜ 0.05).Conclusion This study demonstrates that the Res could alleviate liver damage and that the Res plays a beneficial role to resist inflammation and apoptosis by activating the SIRT1 which probably inhibits the expression of NF-κB protein in cholestatic liver injury.The Res also shows antioxidant effect by promoting the expression of PPARα. Key words: Resveratrol; Cholestasis; Silent information regulator 1 ; Peroxisome proliferator activated receptor-alpha; Nuclear factor-κBp56