Abstract
We examined the immunogenicity and the protection by a recombinant blood stage antigen derived from the malaria parasite Plasmodium falciparum SERA (serine-repeat antigen) in squirrel monkeys ( Saimiro sciureus). The monkeys were immunized with recombinant SE47' protein produced using a synthetic gene expressing amino acids 17-382 (SE47') of the SERA polypeptide in E. coli, with or without Freund's adjuvant. All monkeys administered with SE47' protein developed the antibody. After the challenge by the blood stage P. falciparum (Indochina-1 strain), the mean parasitemia in a group of monkeys immunized with SE47' protein in the absence of the adjuvant was lower than that of the control group, especially in an early phase of the infection. Antibody titers against SERA protein in two of the SE47' monkeys immunized without Freund's adjuvant were stimulated after the parasite challenge. The observed increase in SERA ELISA titers following challenge infection correlated with the degree of protection as assessed by significant reductions in peak levels of parasitemia. In addition, immune serum from SE47' immunized monkeys inhibited the growth of P. falciparum in vitro. These results demonstrate that protective immune responses can be induced by an E. coli-produced recombinant SE47' SERA proteins in Saimiro sciureus monkeys, and further that the humoral immune response after experimental immunization can be boosted by P. falciparum challenge infection, presumably due to native SERA molecules produced by challenge parasites.
Published Version
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