Protective efficacy of Tephrosia purpurea against Aflatoxin B1 induced oxidative damage in rats

Abstract

Tephrosia purpurea (TP), commonly known as wild indigo, has a unique quality of healing all kinds of wounds and it is also an important ingredient of many Ayurvedic formulations. Thus, the present investigation was designed to validate and evaluate protective efficacy of ethanolic extract of Tephrosia purpurea (TP) against Aflatoxin B1 (AFB1) induced renal dysfunction. The ethanolic extract of Tephrosia purpurea was prepared by Soxhlet extraction method. For present study female rats (Wistar strain) were randomly divided into 6 groups with 6 animals in each group. The entire regime was of 28 days. AFB1 was administered at 200 µg/kg dose and TP was administered at three different doses (100, 200 and 300 mg/kg). 24 hours after last treatment the animals were euthanised various biochemical test were performed. AFB1 exposure significantly elevated uric acid, urea, creatinine in serum and lipid peroxidation in renal tissue indicating cellular damage. On the other hand, decline in activities of superoxide dismutase, catalase and reduced glutathione suggests exhaustion of endogenous defence system. Treatment with TP extract offers protection at all the three doses (100, 200 and 300 mg/kg). Although, maximum recovery was seen with 300 mg/kg dose of TP.