Protective effects ofsevoflurane pretreatment on LPS-induced acute lung injury in rats

Abstract

：Objective To investigatethe effects of sevoflurane pretreatment on Lipopolysaccharide (LPS)-induced acute lunginjury (ALI) in rats. Methods Seventy -two Sprague -Dawley rats were randomly divided into6 groups: NS group, LPS group and sevoflurane pretreatment (S-l h group, S-6 h group, S-12h group and S-24 h group). The rat model of ALI was established by intratrachealinstillation of LPS. Animals were sacrificed at 6 h after LPS or NS administration.Leukocyte count, concentration of TNF-αand IL-β in bronchoalveolar lavage fluid (BALF); pulmonary capillarypermeability, myeloperoxidase (MPO) activity of lung tissue; lung histological changeswere compared in rats with or without sevoflurane pretreatment (2.4% inspired for 30 min)at different times before LPS instillation. Results Compared with the NS group,severeinjury of lung tissues and increase in leukocyte count in BALF, Production of TNF-α and IL-1β in BALF, pulmonary capillarypermeability and MPO activity in the lung were significantly increased in rats treatedwith LPS (P<0.01). MPO activity, leukocyte count and production of IL-1βinBALF were reduced when sevoflurane was given 1 or 24 h before but not at 6 or 12 h beforeLPS instillation(P<0.01). Sevoflurane pretreatment also attenuated pulmonary capillarypermeability and production of TNF-α in BALF (P<0.01).Pulmonary capillary permeability and concentration of TNF-α in S-l h and S-24 h group was lower than S-6 h and S-12 hgroup (P<0.01). Sevoflurane pretreatment was effective at 1 h and 24 h suggestingsevoflurane has early and late protection against LPS-induced lung injury. ConclusionSevoflurane pretreatment has protective effects against acute lung injury when given 1 or12 h before LPS instillation. Key words: Acute lunginjury; Sevoflurane; Lipopolysaccharides; Cytokines