Protective effects ofAcanthopanax koreanum Kakaiextract against carbon tetrachloride-induced liver injury in Sprague-Dawley rats

Abstract

Purpose: This study was conducted in order to investigate the protective effects of ethanolic extract of Acanthopanax ko-reanum Nakai (AE) against carbon tetrachloride (CCl₄)-induced liver injury in rats. Methods: Male Sprague-Dawley rats were randomly divided into four groups in order to receive the following experimental diets with intraperitoneal injection of CCl₄ (2.0 mL/kg body weight, 20% solution 0.65 mL) for eight weeks (n = 8 per group): CCl₄ control (CON), CCl4 + AE 1% (AE1), CCl₄ + AE 3% (AE3), or CCl₄ + acanthoic acid 0.037%, which is equivalent to AE 3% (AA). Results: Highest serum ALT activity and albumin level were observed in the CCL₄ control group, but showed a significant decrease by either AE or AA supplementation in a dose-dependent manner (p = 0.0063 and 0.0076, respectively). Both hemotoxylin and eosin staining and Masson’s staining indicated remarkable prevention of CCl₄-induced liver damage in the AE3 group. TNFα and IL-6 production were significantly lowered in the AE treated groups, but not in the AA group (p = 0.0016 and p = 0.0002, respectively). The effects of AE3 were greater than those of AA for inflammation and liver toxicity biomarkers. Con-clusion: Taken together, the results suggested that ethanolic extract of Acanthopanax koreanum Nakai provided hepa-toprotective effects, leading to the reduction of inflammatory response. In addition, the effect of AE was superior to that of single compound AA.