Protective effects of vitamin B12 against cisplatin induced nephrotoxicity in adult mice

Abstract

Cisplatin (CP) is one of the most widely used and highly effective cytotoxic agents in the treatment of various cancers, but its clinical use is associated with considerable nephrotoxicity. This characteristic of CP makes it harmful to the cancer patient's kidneys. In the present study, we report the effects of vitamin B12 on rat kidneys treated with cisplatin using single doses of both compounds. CP was administered to adult albino mice at a dose of 1 mg/kg of body weight for 10 days. CP caused oxidative stress by significantly (p∠0.01) suppressing the expressions of NRF-2 and HO-1, followed by the upregulation (p∠0.01) of inflammatory markers like NF-kB, TNF-α and IL-1β proteins in the kidney's homogenates. The co-administration of vitamin B12 at a dose of (500 mg/kg) significantly (p∠0.01) reduced the oxidative stress (activated NRF-2 and HO-1 proteins) mediated by inflammatory mediators (NF-kB, TNF-α, and IL-1β proteins) to decrease the intensity of the toxicity in the kidneys of the adult albino mice. The results suggest that vitamin B12 is an effective chemoprotective agent against nephrotoxicity induced by the antitumoral cisplatin in adult mice.