Abstract
BackgroundHordeum vulgare L (barley) contains numerous phenolic substances with proven anticancer, antioxidant and gastroprotective activities. Saccharification increases the functionality and bioavailability of these compounds thus can aid in the development of a natural product based medicine. This study aimed to investigate the possible gastroprotective effects of saccharification on the indomethacin (IND)-induced gastric ulcers in rats using Weissella cibaria- and Saccharomyces cerevisiae-triple fermented H. vulgare extract (FBe).MethodsIn total, 60 healthy male 6-week old Sprague-Dawley SD (SPF/VAF Outbred CrljOri:CD1) rats were commercially purchased. The FBe extract (100, 200, and 300 mg kg− 1) was orally administered 30 min before an oral treatment of IND (25 mg kg− 1). Six hours after IND treatment, variations in the histopathology, myeloperoxidase (MPO) activity, gross lesion scores, lipid peroxidation, and antioxidant defense system component (superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH)) levels were measured.ResultsFBe treatment showed significant (p < 0.01 or p < 0.05) and dose-dependent decrease in gastric mucosal damage. In the present study hemorrhagic gross lesions, gastric MPO activity, and histopathological gastric ulcerative lesions were observed in IND-treated rats compared to the IND control rats. In particular, FBe, in a dose-dependent manner, strengthened the antioxidant defense systems, decreased lipid peroxidation and CAT activity by increasing the GSH levels and SOD activity, respectively. The 200 mg kg− 1 dose of FBe was similarly gastroprotective as the 10 mg kg− 1 dose of omeprazole in rats with IND-induced gastric mucosal damage.ConclusionsThe findings of the present study show that an oral administration of FBe had positive gastroprotective effects through strengthening the body antioxidant defense system and anti-inflammatory effects.
Highlights
Hordeum vulgare L contains numerous phenolic substances with proven anticancer, antioxidant and gastroprotective activities
Insignificant restricted ulcerative lesions were observed in intact control rats while focal hemorrhagic ulcerative lesions in IND treated rats were grossly dispersed in the gastric mucosa
Marked inhibitions of the gross gastric damages were noticed in OM (10 mg kg− 1) and fermented barley extract (FBe) (100, 200, and 300 mg kg− 1) treated rats
Summary
Hordeum vulgare L (barley) contains numerous phenolic substances with proven anticancer, antioxidant and gastroprotective activities. This study aimed to investigate the possible gastroprotective effects of saccharification on the indomethacin (IND)-induced gastric ulcers in rats using Weissella cibaria- and Saccharomyces cerevisiae-triple fermented H. vulgare extract (FBe). Various synthetic anti-ulcer drugs such as misoprostol are used to cure NSAID induced gastric ulcers. Indomethacin (IND) is widely approved in medical practice as being an NSAID; it shows exceptional efficiency in the treatment of fever, pain and inflammation by suppressing the synthesis of prostaglandins through inhibiting the cyclooxygenase enzymes [7]. NSAIDs such as IND administration result in gastrointestinal tract infections due to the inhibition of prostaglandin synthesis [8]. The free radical scavenging property of synthetic drugs might have a protecting effect against gastric mucosal oxidative damage that accelerates healing of gastric ulcers [10]. Disorder of gastric mucosal antioxidant defense system has been associated with NSAIDs [11]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
