Protective Effects of the Hydroethanolic Extract of Fridericia chica on Undifferentiated Human Neuroblastoma Cells Exposed to α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL).

Neda Alvarez-Ortega,Karina Caballero-Gallardo,Cristina Juan,Elena E Stashenko,Jesus Olivero-Verbel,María Taboada-Alquerque,Jackeline Franco,Ana Juan-García

doi:10.3390/toxins13110748

Abstract

Fridericia chica (Bignoniaceae) is a traditional medicinal plant. The aim of this research was to determine the protective effects of the hydroethanolic extract from the F. chica leaves (HEFc) against the cytotoxicity of zearalenone (α-ZEL) and β-ZEL on SH-SY5Y cells. Free radical scavenging activity of HEFc was evaluated using the DPPH method. The cytotoxicity of both zearalenone metabolites and HEFc was examined using MTT test, as was the cytoprotective effects of the HEFc on cells treated with these mycotoxins. The chemical composition of HEFc was determined using UPLC-QTOF-MS/MS. HEFc elicited good DPPH radical scavenging activity following a concentration-dependent relationship. Cells exposed to α-ZEL exhibited a viability ˂50% after 48 h of treatment (25 and 50 µM), while those exposed to β-ZEL showed viability ˂50% (100 µM) and ˂25% (25-100 µM) after 24 and 48 h of exposure, respectively. HEFc showed a significant increase in cell viability after exposure to α-ZEL (25 and 50 µM) and β-ZEL (6–100 µM) (p < 0.05). UPLC-QTOF-MS/MS analyses allowed the identification of 10 phytochemical components in the HEFc. In short, the hydroethanolic extract of F. chica grown in Colombian Caribbean can protect against the effects of mycotoxins and it is a valuable source of compounds with antioxidant properties.

Highlights

Fridericia chica leaves are usually employed as a red dye, it has traditionally been used by Sinu artisans of the Colombian Caribbean to make the vueltiao hat [2]
The aim of this work was to investigate the potential of a hydroethanolic extract of F. chica (HEFc) grown in northern Colombia to mitigate the effects of α-ZEL and β-ZEL in undifferentiated human neuroblastoma, as well as to characterize its constituents
This study evaluated the free radical scavenging activity of a hydroethanolic extract

Summary

Introduction

The red color obtained from F. chica comes from anthocyanidins, a class of phenolic compounds with known antioxidant properties [3], and extracts from the plant have shown pharmacological activities linked to beneficial health effects, including anti-inflammatory [4], antiproliferative [5], wound healing [6], antispasmodic [7], photoprotective [8], and leishmanicidal [9] activities Many of these properties are attributed to different flavonoid compounds reported in F. chica, such as isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, apigenin, and hispidulin [10], this last a potential compound for neuroinflammation inhibition [11]

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Methods

Conclusion