Abstract
Abstract: Objective: To investigate whether the extract of Juglans mandshurica maxim could inhibits the apoptosis induced by endogenous H2O2 on Endothelial Cell （EVC-304）in vitro. METHODS: Cultured EVC-304 cells were incubated with 10mUGOX or with 10mUGOX and different concentrations (25μg/ mL, 50μg/ mL, 100μg/ mL) of the extract of Juglans mandshurica maxim for 24h.The proliferation of EVC-304cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The early apoptotic percent was measured by flow cytometry (FCM). RESULTS: MTT results showed that the inhibition proliferation of EVC-304 cells induced by endogenous H2O2 could be inhibited by the extract of Juglans mandshurica maxim in a dose -dependent manner. FCM assay indicated that, after treatment on EVC-304 cells with endogenous H2O2, the early apoptotic percent was increased, but the apoptosis rate was decreased significantly when the extract of Juglans mandshurica maxim added. CONCLUSION: the extract of Juglans mandshurica maxim protected significantly the cell damage and cell apoptosis induced by endogenous H2O2.
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