Abstract
The aim of this study was to investigate the effects of the combination of sodium ferulate (SF) and oxymatrine (OMT) on mice with cecal ligation and puncture (CLP)-induced sepsis. Swiss male mice were randomly divided into a control group, CLP group, three SF + OMT groups (3.1+6.9; 6.2+13.8 and 12.3+27.7 mg/kg), SF (6.2 mg/kg) group and OMT (13.8 mg/kg) group. Eight hours after the administration of the drugs, the survival rates and survival times of the animals were monitored. In addition, the lung wet/dry weight (W/D) ratio; alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in the serum; the C-reactive protein (CRP), interleukin-6 (IL-6) and interferon-γ (IFN-γ) levels in the serum and lung and liver homogenates; and the malondialdehyde (MDA) and superoxidase dismutase (SOD) levels in the lung and liver homogenates were measured. The bacterial load in the serum was also studied. Following treatment with the combination of SF and OMT, the survival rate increased and the survival time was prolonged; CLP-induced increases in the lung W/D ratio and the levels of ALT, AST, LDH, CRP, IL-6, IFN-γ and MDA were significantly reduced; and the SOD activity levels were increased, compared with those of the untreated animals with CLP-induced sepsis. These results indicated that the combination of SF and OMT induced protective effects against CLP-induced lethal sepsis of mice. The possible mechanism of these effects may be associated with the alleviation of systemic inflammation and diminishment of oxidative injury.
Highlights
Sepsis, a systemic inflammatory response induced by severe infection, which usually leads to multiple organ dysfunction syndromes, is a common cause of critical illness and mortality in intensive care units [1]
The present study used the cecal ligation and puncture (CLP)‐induced model of sepsis to further corroborate the anti‐inflammatory effect of the combination of sodium ferulate (SF) and OMT and aimed to demonstrate its protective effect on sepsis‐induced organ injury
Sepsis resulting from CLP in animals is an accepted animal model that closely imitates the physiological changes observed during the progression of sepsis in humans [18]
Summary
A systemic inflammatory response induced by severe infection, which usually leads to multiple organ dysfunction syndromes, is a common cause of critical illness and mortality in intensive care units [1]. In 2007, the severe sepsis mortality rate was reported to range between 30 and 50%, rising to 80‐90% for patients with septic shock and multiple organ failure [4]. In the progression of sepsis, it is considered that the hyperactive systemic inflammatory response, with a large number of inflammatory cytokines and excessive generation of free radicals, is one of the main causes of multiple organ injury. It may be hypothesized that treatment with a combination of SF and OMT will alleviate the inflammatory response and multiple organ injury induced by sepsis
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