Protective effects of Teucrium polium aqueous extract and ascorbic acid on hematological and some biochemical parameters against carbon tetrachloride (CCl4) induced toxicity in rats

Abstract

Herbal drugs play a crucial function to protect organisms from the toxic effect of some compounds. The present study was designed to evaluate the protective effects of Teucrium Polium (TP) aqueous extract and vitamin C (Vit C) against carbon tetrachloride (CCl4) which induced toxicity in rats. Male albino Wistar rats were divided into six groups: group I was used as controls, group II received CCl4 in olive oil (0.5ml/kg) by gavage, and group III received CCl4 in olive oil (0.5ml/kg) by gavage after three days of receiving TP (5g/l), orally, for seven days; Group IV received TP (5g/l) alone, orally, for seven days, Group V received CCl4 in olive oil (0.5ml/kg) by gavage after three days of receiving Vit C (250mg/kg) by intramuscular injection and Group VI received Vit C (250mg/kg) alone by intramuscular injection, for day seven. Some biochemical and hematological parameters were investigated. Our results showed that the administration of CCl4 caused hepatotoxicity as monitored by the significant increase in the levels of hepatic markers enzymes (lactate dehydrogenase (LDH), Alkaline phosphatase (PAL), total bilirubin (TB) and Gamma glutamyl transferase (γGT) levels) and a decrease in hematological parameters such as white blood cell (WBC), red blood cell count (RBC), mean corpuscular hemoglobin concentration (MCHC) and blood platelet count (PLT). Treatment with TP or Vit C appeared to be effective against hematotoxic and the liver changes induced by CCl4, as evidenced by the improvement of the parameters cited above. The CCl4-treated group demonstrated a significant increase of malondialdehyde (MDA) levels in erythrocytes thus causing a reduction in antioxidant defense system. Pretreatment with TP or Vit C improved the biochemical analyses, hematological parameters, and antioxidant defense system.