Abstract
Effects of sulfhydryl compounds on cell injuries caused by hypochlorous acid (HOCl) were studied in isolated rat cardiomyocytes by way of continuous measurement of intracellular Ca 2+ using calcium sensitive fluorescent dye, fura-2. Ten minutes exposure of the cell to 100 μM HOCl induced a significant increase in the intracellular free calcium concentration ([Ca 2+] 1) from 90±20 nM to 266±74 nM ( n=44, mean±S.D.). This increase in [Ca 2+] 1 was reversed by subsequent application of dithiothreitol (DTT) in a dose dependent manner: 30 μM DTT was found effective, while 2 mM DTT almost completely restored the [Ca 2+] 1 to the control level. Similar to DTT, cysteine ethyl ester and cysteine methyl ester could also reverse the HOCl-induced rise of [Ca 2+] 1 where the order of the potency was DTT > cysteine ethyl ester > cysteine methyl ester. In contrast, increase in [Ca 2+] 1 induced by HOCl was not recovered by application of other sulfhydryl compounds such as cysteine and glutathione. Since HOCl administrated in the presence of cysteine failed to induced an [Ca 2+] 1 we conclude that membrane permeable sulfhydryl compounds may reverse the increase in [Ca 2+] 1 caused by HOCl.
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