Protective effects of stilbene glycoside on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine induced mice model of Parkinson's disease

Abstract

Objective To investigate the protective effects of stilbene glycoside(2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside, TSG)on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP)-induced mice model of Parkinson's disease(PD). Methods Mice were randomly divided into the blank control group, the negative control group, the TSG high-dose group, the TSG low-dose group and the positive drug group(n=20 each). Mice were weighted daily to observe the changes of body weight, and mice motor and behavior function were tested by open field test.Level changes of α/β synuclein in brain cortex, cerebellum, midbrain, and hippocampal were detected by Western blot. Results Compared with the blank control group, the negative control group showed that the body weight was decreased(P<0.05). Compared with the negative control group, the body weight was increased in the TSG high-and low-dose groups and the positive drug group(P<0.05). The spontaneous behavior was impaired in the negative control group.Compared with the blank control group, the negative control group showed that the open field test showed traveled distance over a 10-min period was significantly shortened at 1st, 7th, 28th days after testing(all P<0.05). The trajectory of motor axons indicated that mice in the negative control group showed dyskinesia, but the groups of positive drug and high-and low-dose of TSG could reverse this dyskinesia.Compared with the blank control group, brain α/β synuclein protein levels were increased in the negative control group, and decreased in positive drug and TSG high-and low-dose groups(P<0.05). Conclusions Stilbene glycosides exert neuroprotective effects in MPTP-induced mice model of PD. Key words: Parkinson disease; Stilbenes; 1-methy-4-phenyl-1, 2, 3, 6-tetrahydropyridine