Protective Effects of Rhino-Hepato Forte in Broiler Chickens During Aflatoxicosis

Abstract

A research was conducted to investigate the counteracting effect of Rhino-Hepato (RHF), against aflatoxin B1 effect on the hematology, serum biochemical parameters and its immunomodulatory effect on Newcastle disease antibody titer in broilers. One hundred twenty , of one day old commercial Ross 308 broiler chicks were divided into four groups: G1 [Control group, without addition of Aflatoxin (AF) or (RHF) ; G2 [ feed contaminated with 500ppb AF]; G3 [feed contaminated with 500ppb AF + 1 ml / L of RHF in drinking water ]; G4 [feed contaminated with 500ppb AF + 2 ml/L of RHF in drinking water]. Birds were vaccinated with Newcastle and lasota vaccines at 8 and 28 days of age. At the end of the experiment (42 days), 72 birds were slaughtered and blood samples from them were collected for evaluation of (RHF) counter effect against aflatoxin B1. Results showed that AFB1 was negatively affecting hematological parameters by reducing RBCs, PCV and Hemoglobin, WBCs and increasing H/L stress factor ratio. AFB1 also had a deleterious effect on serum biochemistry by increasing triglycerides , cholesterol, AST, ALP and creatinine, while reducing glucose, total protein and calcium. Antibody titers against NDV vaccine were significantly reduced by contaminating feed with AFB1. Inclusion of (RHF) at two doses (1 and 2 ml/L) was responsible for restoring all the previous parameters to those of the normal ones. It is concluded that(RHF), containing silymarin and other active ingredients protect birds against adverse effects of (AFB1) one hematological, biochemical and NDV antibody titers.