Abstract
Oxidative stress-induced retinal ganglion cell (RGC) death is one of the important factors in ocular disease such as glaucoma. The aim of this study is to investigate whether protocatechuic acid (PCA), a monomeric phenolic acid with strong free radical scavenging effects, could protect RGC from oxidative stress induced by H2O2 and provide a potential therapy drug. The viability of RGC was determined by the MTT reduction and lactate dehydrogenase (LDH) assay. The possible protective mechanism was investigated via detecting apoptosis by Hoechst staining, Bcl-2 and Bax protein levels by ELISA, caspase-3 activation and membrane potential by immunofluorescence, and caspase-3 mRNA expression by polymerase chain reaction (PCR) method. The results showed that PCA was effective in reducing apoptotic death induced by oxidative stress, mainly through inhibiting depolarization of membrane and activation of caspase-3, down-regulating of apoptosis-related protein Bax, and up-regulating Bcl-2.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
