Abstract
The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP’s ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent.
Highlights
IntroductionExposure to ionizing radiation inflicts single strand breaks, double strand breaks, base damage and DNA-protein cross-links in the genomic DNA [1]
Ionizing radiation causes a variety of biological effects in living cells
X-ray radiation treatment significantly inhibited the growth of the mouse spleen lymphocytes
Summary
Exposure to ionizing radiation inflicts single strand breaks, double strand breaks, base damage and DNA-protein cross-links in the genomic DNA [1]. Search for the chemical or biological agents that are able to protect human beings from the ionizing radiation is a key issue in radiation biology. Numerous drugs of both synthetic and natural origin, e.g., antioxidants [3,4], sulfhydryl compound, estrogens, haemopoietic growth factors and cytokines [5] have been tested in both in vitro and in vivo models to mitigate injuries caused by ionizing radiation. Amifostine (Ethyol®) is the only radio-protector that has been approved by the Food and Drug
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