Abstract
The objective of present study was to investigate the antioxidative and cardioprotective effects of polyphenol extracts from sea buckthorn (PESB) against myocardial ischemia reperfusion injury (MIRI). PESB was analyzed using HPLC-DAD and their antioxidant activities were evaluated by measuring the intrinsic free radicals cavenging capacity and inhibiting the formation of hydroxyl radical capacity. Experiments on the isolated rat hearts were carried out according to the improved Langendorff retrograde perfusion technology. Coronary effluent of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) activities were analyzed to determine the extent of myocardial injury. Protein expression levels of Beclin-1, microtubule-associated protein 1 light chain 3 (LC3) I and II were measured to evaluate the degree of autophagy. In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that PESB has potential health implications in both prevention and amelioration of MIRI, which may be attributed to the inhibition of autophagy.
Highlights
Sea buckthorn (Hippophaė rhamnoides L.) is a thorny nitrogen fixing deciduous shrub, naturally distributed throughout Asia and Europe [1]
In this work, we studied the polyphenol extracted from sea buckthorn using ultrasonic-assisted 70% ethanol extraction technology to verify the relation between inhibition of autophagy and myocardial ischemia reperfusion injury (MIRI) under ischemia reperfusion (I/R) conditions on the isolated rat heart
Chemical analysis of polyphenol extracts from sea buckthorn (PESB) by HPLC revealed the presence of the following compounds: gallic acid, proto catechuic acid, (+)-catechin, caffeic acid, chlorogenic acid, ferulic acid, p-coumaric acid, salicylic acid, cinnamic acid, quercetin, phlorizin, rutin, kaempferol and isorhamnetin
Summary
Sea buckthorn (Hippophaė rhamnoides L.) is a thorny nitrogen fixing deciduous shrub, naturally distributed throughout Asia and Europe [1]. (2016) Protective Effects of Polyphenol Extracts from Sea Buckthorn (Hippophaė rhamnoides L.) on Rat Hearts. Many research results have pointed out that berries, seeds and oil of sea buckthorn are food-derived medicinal and nutritional sources could serve as natural remedies for the reduction in risk of cardiovascular disease and other health related problems such as tumor, stomach ulcer and skin disease [6] [7]. Cardiovascular disease (CVD) is one of the most serious causes of mortality and morbidity of people all over the world [8]. Successful reperfusion therapy for CVD can attenuate myocardial infarction and prevent heart failure, leading to reduce mortality and morbidity [10]
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