Abstract
BackgroundMassive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect of Citrus sinensis peel aqueous extract (CSPE) and Hesperidin (H) in ethanol (EtOH) induced oxidative stress and peptic ulcer in rats.MethodsSeventy adult male Wistar rats were divided into seven groups of 10 each: control, EtOH (4 g/kg b.w.), EtOH + various doses of CSPE (100, 200 and 400 mg/kg, b.w.), EtOH + Hesperidin (50 mg/kg, p.o.) and EtOH + Omeprazole (OM, 20 mg/kg, p.o.). Animals were perorally (p.o.) pre-treated with CSPE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) during 2 h. Gastric ulcer was induced in rats with a single dose of ethanol (EtOH). Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), malondialdhyde (MDA), hydrogen peroxide H2O2 and Thiol groups (−SH) content in stomach and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and gluthation peroxidise (GPx) were measured. Furthermore, histopathological examinations were performed.ResultsThe results showed that ethanol induced gastric damage, improving oxidative stress markers level such as MDA (121 ± 4.45 nmol/mg proteins) and H2O2 (24.62 ± 1.04 μmol/mg proteins), increased pro-inflammatory cytokine (TNF-α level), as well as the expression of COX-2 in the ethanol group. However, a significant depletion of enzymatic and non-enzymatic antioxidants were observed, such as, GPx (72%), SOD (57.5%), CAT (41.6%) and -SH (50%). The lesions were associated with severe histopathological damage. The both Citrus sinensis peel aqueous extract (CSPE) and hesperidin significantly protect against all gastric damages caused by ethanol administration in rats.ConclusionsWe propose that CSPE and hesperidin exhibit protective effects in EtOH-induced peptic ulcer in rat. This protection might be related in to part its antioxidant properties as well as its opposite effects on some studied intracellular mediators.
Highlights
Massive alcohol drinking can lead to gastric ulcer
A similar protective effect had observed with Omeprazole-induced a significant reduction in gastric TNF-α compared to treat ulcerated rats (P < 0.05)
Effect of Citrus sinensis peel aqueous extract (CSPE) and hesperidin on EtOH-induced gastric lipoperoxidation and hydrogen peroxide increase Bearing on the effect of EtOH, CSPE and hesperidin on oxidative stress condition, we firstly studied the gastric lipoperoxidation and hydrogen peroxide content (Fig. 5)
Summary
Massive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect of Citrus sinensis peel aqueous extract (CSPE) and Hesperidin (H) in ethanol (EtOH) induced oxidative stress and peptic ulcer in rats. Gastric ulcers affect annually approximately 4 million people in the globe [1, 2] This pathology is a heterogeneous disease of multifactorial etiology which can result from alcohol consumption, smoking, Helicobacter pylori infections, use of anti-inflammatory drugs (NSAIDs) [3]. This condition affects approximately 10-15% of the population in the world [4]. The production of ROS plays a major role in the apparition and development of stomachal pathologies, such as gastric adenocarcinoma, peptic ulcerations, or gastritis [6] In this respect, gastric mucosal layers represent a dynamic barrier in counteracting the effects of noxious agents through series of endogenous antioxidant defense systems. The mechanisms underlying the ethanol-induced gastric and duodenal injuries have not yet been fully elucidated
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