Abstract
ObjectivesOmega‐3 fatty acids are well‐known unsaturated fatty acids that are essential for growth and development in animals. They primarily participate in the development of intelligence, the nervous system, and vision, and the metabolism of neurotransmitters. Omega‐3 fatty acids have been widely studied in the treatment of Alzheimer's disease (AD). Omega‐3 fatty acids are known to have neuroprotective effects due to their antioxidant capacity. Rotenone has been shown to induce neurotoxicity in vitro.MethodsWe investigated the protective effects of omega‐3 fatty acids against AD in rat brain microvascular endothelial cells (RBMVECs) in vitro. Lipid peroxidation, reactive oxygen species (ROS), glutathione peroxidase (Gpx), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase levels were evaluated in RBMVECs. Flow cytometry was performed to assess apoptosis.ResultsLipid peroxidation and ROS were reduced in RBMVECs following incubation with omega‐3 fatty acids. Catalase, Gpx, and SOD were increased in RBMVECs following incubation with omega‐3 fatty acids. Flow cytometry showed that incubation with omega‐3 fatty acids reduced the amount of apoptotic RBMVECs.ConclusionOur results suggest that omega‐3 fatty acids show potential as a therapeutic agent against AD.
Highlights
Alzheimer’s disease (AD) is a well-known chronic neurodegenerative disease characterized by major dementia (Burns & Iliffe, 2009)
We investigated the in vitro protective effects of omega-3 fatty acids against AD in rat brain microvascular endothelial cells (RBMVECs)
Omega-3 fatty acids, fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium, sulforhodamine B (SRB), dimethyl sulfoxide, trypsin- EDTA, 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and penicillin- streptomycin were obtained from Sigma-Aldrich (Sigma-Aldrich China, Inc., Shanghai, China)
Summary
Alzheimer’s disease (AD) is a well-known chronic neurodegenerative disease characterized by major dementia (Burns & Iliffe, 2009). Rotenone is a well-known pesticide that is present in plants. Such as the Fabaceae and the jicama vine. Elevated levels of reactive oxygen species (ROS) have been associated with aging, diabetes. Omega-3 fatty acids have been reported to react with aldehydes to protect cellular proteins. This suggests that omega-3 fatty acids have potential utility as potent therapeutic agents against rotenone-induced neurotoxicity. We investigated the in vitro protective effects of omega-3 fatty acids against AD in rat brain microvascular endothelial cells (RBMVECs)
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