Protective Effects of Micronized Fat against Ultraviolet B–Induced Photoaging

Abstract

Sir: We read with great interest the article entitled “Protective Effects of Micronized Fat against Ultraviolet B–Induced Photoaging” by He et al. in Plastic and Reconstructive Surgery.1 The authors conducted in vitro and in vivo experiments to prove the content and effects of micronized fat. The authors found that the micronized fat produced more adipose-derived mesenchymal stem cells than control fat. However, mechanical manipulation would disrupt adipose-derived mesenchymal stem cell structure and lead to a decrease in cell viability. Thus, we believe it is necessary to further detect cellular viability such as proliferation and differentiation potential. Osinga et al.2 reported the characteristics of shuffling lipoaspirated fat, suggesting that intersyringe processing does not alter the tissue viability and microscopic structure. We would like to know what the difference is between the method applied in this study and that reported by Osinga et al.2 In addition, what considerations are the authors basing their use of epididymal fat on instead of inguinal fat in animal experiments? The inguinal fat was used more in the literature because of a wide range of sources. The authors performed an in vivo study to assess the effects of micronized fat on ultraviolet B–induced photoaging. We think it is necessary for the authors to prove that the photoaging model was successfully made before fat injection. Also, it is necessary for the authors to report the injection dose they used, which would influence the effect of fat. Because the skin thickness of the back is different between rats, we suggest that the authors explain how to eliminate the influence of the rat skin itself on the results. Lastly, we want to know why the experiment of fat graft survival is not performed in the experiment of photoaging to minimize the number of animals used. DISCLOSURE The authors have no financial interest to declare in relation to the content of this communication. No funding was received for this communication. Zuguang Hua, M.D.Peng Wei, M.D., Ph.D.Ningbo No. 1 HospitalHaishu DistrictNingbo, Zhejiang, People’s Republic of China