Abstract
A previous study has shown that tilapia fish skin gelatin hydrolysates inhibited photoaging in vivo, and that, Leu-Ser-Gly-Tyr-Gly-Pro (LSGYGP) identified in the hydrolysate had a high hydroxyl radical scavenging activity. In this study, activities of LSGYGP were further evaluated using ultraviolet B (UVB)-induced mouse embryonic fibroblasts (MEFs). UVB irradiation significantly increased the intercellular reactive oxygen species (ROS) production and matrix metalloproteinases (MMPs) activities and decreased the content of collagen in MEFs. LSGYGP reduced the intercellular ROS generation in UVB-induced MEFs. Meanwhile, the decrease of superoxide dismutase (SOD) activity and the increase of malondiaidehyde (MDA) content were inhibited by LSGYGP. LSGYGP reduced MMP-1 and MMP-9 activities in a dose-dependent manner. Molecular docking simulation indicated that LSGYGP inhibited MMPs activities by docking the active sites of MMP-1 and MMP-9. Furthermore, LSGYGP also affected the intercellular phosphorylation of UVB-induced the mitogen-activated protein kinase pathway. LSGYGP could protect collagen synthesis in MEFs under UVB irradiation by inhibiting oxidative stress and regulating MMPs activities.
Highlights
Ultraviolet (UV) irradiation, including ultraviolet A (UVA) (315–400 nm) and ultraviolet B (UVB)(290–320 nm), can generate reactive oxygen species (ROS)/nitrogen species
We aim to study the protective effects of LSGYGP on intercellular collagen formation, ROS generation and matrix metalloproteinases (MMPs) levels in UVB-induced mouse embryonic fibroblasts (MEFs)
UVB irradiation significantly decreased the viability of MEFs compared with that of the normal control (NC) group (p < 0.05)
Summary
Ultraviolet (UV) irradiation, including ultraviolet A (UVA) (315–400 nm) and ultraviolet B (UVB). (290–320 nm), can generate reactive oxygen species (ROS)/nitrogen species. Excessive oxidative stress can cause photoaging and even apoptotic or necrotic skin cell death [1]. UVA, UVB irradiation produces greater biological effects. UVB may pass through the epidermis, penetrate the upper part of the dermis, and induce the generation of excessive ROS [2]. The matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases and can degrade all components of extracellular matrix protein and connective tissues [3].
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