Abstract
Clostridium perfringens (C. perfringens) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of Lactobacillus plantarum (L. plantarum) Lac16 on C. perfringens infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that L. plantarum Lac16 significantly inhibited the growth of C. perfringens, which was accompanied by a decrease in pH levels. In addition, L. plantarum Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of C. perfringens to IPEC-J2 cells, and attenuated C. perfringens-induced cellular cytotoxicity and intestinal barrier damage. Furthermore, L. plantarum Lac16 significantly suppressed C. perfringens-induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, L. plantarum Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by C. perfringens infection. Collectively, probiotic L. plantarum Lac16 exerts protective effects against C. perfringens infection-associated injury in IPEC-J2 cells.
Highlights
Clostridium perfringens (C. perfringens) is a Gram-positive, spore-forming, anaerobic, rod-shaped bacterium [1,2], which can be isolated from the natural environment and intestines of human and animals as a component of the normal microbial community [3,4]
C. perfringens type A is very common in the intestines of warm-blooded animals; it could cause intestinal diseases in domestic animals, such as pigs, chickens, and sheep [3,10]
In the group of C. perfringens co-incubated with the Lac16 fermentation supernatant (LFS), the OD600 was significantly lower than that of the CP group (p < 0.001), indicating that LFS could inhibit the proliferation of C. perfringens
Summary
Clostridium perfringens (C. perfringens) is a Gram-positive, spore-forming, anaerobic, rod-shaped bacterium [1,2], which can be isolated from the natural environment (e.g., soil) and intestines of human and animals as a component of the normal microbial community [3,4]. C. perfringens type A is very common in the intestines of warm-blooded animals; it could cause intestinal diseases in domestic animals, such as pigs, chickens, and sheep [3,10]. It is worth mentioning that antibiotics play a critical role in the prevention and clinical treatment of diseases caused by C. perfringens [13,14]. Under the situation of feed antibiotics prohibition, C. perfringens infection has become an important problem, hindering the development of the pig industry [13,15,16]. Many feed additives are used in animal husbandry to prevent C. perfringens infection, such as probiotics, prebiotics, symbiotics, essential oils, and herbs [17,18,19,20]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
