Abstract
Long terms and repeated exposure of UVB light on the skin induces up-regulation of matrix metalloproteinases (MMPs). UV-induced MMPs cause connective tissue damage and skin to become aged. The present study was performed to elucidate the anti-aging actions of fucoidan on UV-induced aging in human skin fibroblasts. The HS68 cells were pretreated with fucoidan at doses of 10μ/ml, 25μ/ml, 50μ/ml for 24 h. After UVB irradiation, they were then further incubated. The level of aging were determined by measuring MTT assay, senescence-associated ß-galactosidase (SA- ßG) activity, and ELISA tests. In case of MTT assay, fucoidan treatment showed significant improvement in cell viability in the following order; 50μ/ml>25μ/ml>10μ/ml>100μ/ml(p<0.05). In ELISA test, pretreatment with fucoidan as doses of 10μ/ml, 25μ/ml, 50μ/ml significantly inhibited the production of MMP-1 compared to the UVB only irradiated by 27%, 30%, and 34%, respectively (p<0.05). Additionally, SA- ßG activity was markedly decreased in the presence of fucoidan. Based upon these results, it seems that fucoidan might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. ∗This work was supported by the grant of KOSEF.
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