Protective effects of endothelin-A receptor antagonist BQ123 against LPS-induced oxidative stress in lungs

Abstract

The aim of this study was to assess whether endothelin-A receptor (ETA-R) blocker, BQ123, influences lung edema, lipid peroxidation (TBARS), hydrogen peroxide (H2O2), TNF-α concentration or the glutathione redox system in the lung homogenates obtained from LPS-induced endotoxic shock rats. The study was performed on male Wistar rats (n=6 per group) divided into groups: (1) saline, (2) LPS (15mg/kg)-saline, (3) BQ123 (0.5mg/kg)-LPS, (4) BQ123 (1mg/kg)-LPS. The ETA-R antagonist was injected intravenously 30min before LPS administration. Five hours after saline or LPS administration, animals were sacrificed and lungs were isolated for indices of lung edema, oxidative stress and TNF-α concentration. Injection of LPS alone resulted in lung edema development and a marked increase in TNF-α (p<0.02), TBARS (p<0.02), and H2O2 (p<0.01) concentrations as well as a depletion of total glutathione (p<0.01). Administration of BQ123 (1mg/kg), before LPS challenge, led to a significant reduction in TNF-α and H2O2 concentrations (p<0.05) and elevation of both total glutathione and the GSH/GSSG ratio (p<0.05). However, it did not prevent LPS-induced TBARS increase and lung edema formation. Interestingly, a lower dose of BQ123 was much more effective in decreasing H2O2, TBARS, as well as TNF-α levels (p<0.02, p<0.05, p<0.05, respectively). That dose was also effective in prevention of lung edema development (p<0.01). Taken together, the obtained results indicate that BQ123 is highly effective in decreasing LPS-induced oxidative stress in lungs. Moreover, the dose of 0.5mg/kg of the antagonist showed to be more effective in decreasing free radical generation and lung edema in endotoxemic rats.