Protective effects of endothelin-1 antibody on stress induced lesion of gastric mucosa in rats

Abstract

AIM: To explore the protective effects of endothelin-1 antibody (ET-1Ab) on acute gastric mucosa lesions induced by stress in rats. METHODS: Stress ulcer induced by cold-restraint-stress (CRS) was used as model in this study, different dose ET-1Ab were administered by left femoral vein prior to stress so as to observe the effects of the drug on ET-1 levels in plasma and gastric mucosa, gastric mucosa blood flow (GMBF)and ulcer index(UI) of CRS rats. RESULTS: (1) Compared with the normal control group, ET-1 levels of plasma and gastric mucosa and UI were increased significantly in the stress groups (116.2±24.7 mv and 125.1±24.2 mv vs 49.1±9.7 mv, 113.8±29.3 mv and 122.9±19. 6 mv vs 52.3±10.3 mv, 28.6±1.8 mv and 51.2±5.9 mv vs 0, P <0.01), while GMBF were decreased markedly in stress groups (227.8±13.5 mv and 150.8±11.5 mv vs 405.8±23. 3 mv, P <0.01).There was significantly positive correlation between ET-1 and UI (r =0.96, P <0.01), and significantly negative correlation between ET-1 and GMBF(r = -0.91, P <0.01). (2) However, compared with the single-stress groups, ET-1 levels of plasma and gastric mucosa and UI were decreased significantly in ET-1Ab-stress groups (69.2±7.3 mv vs 116.2±24.7 mv, 80.6±12.3 mv vs 125.1±24.2 mv, 58.5±6.3 mv vs 113.8±29.3 mv, 68.9±9.6 mv vs 122.