Abstract
Advanced glycation end products (AGEs) have been regarded as a pivotal inducer in diabetes and kinds of diabetic nephropathy. The present studies explored the effects of Danggui Buxue Tang (DBT) that is a Chinese medicinal de- coction on negative charge to Human Umbilical Vein Endothelial Cell (HUVEC) and the related mechanism. Alcian blue staining was established to evaluate the intensity of negative charge on HUVEC. Proteoglycan expressions of AGP and avidin were determined by SDS-PAGE. We observed that DBT can significantly increase negative charge on HU-VEC and up-regulated AGP and avidin expressions and ameliorate AGEs-induced HUVEC apoptosis. Therefore, all results showed DBT had prevention effects against the progression of AGEs-induced damage, and this decoction might be promising agent against proteinuria in diabetic nephropathy.
Highlights
The World Health Organization estimates that the number of people in the world with diabetes has increased dramatically over recent years and is expected to reach 300 million by the year 2025 [1]
We observed that Danggui Buxue Tang (DBT) can significantly increase negative charge on Human Umbilical Vein Endothelial Cell (HUVEC) and up-regulated acid glycoprotein (AGP) and avidin expressions and ameliorate Advanced glycation end products (AGEs)-induced HUVEC apoptosis
Alpha-1-acid glycoprotein (AGP) and avidin expression were significantly decreased in model group compared with control group (P < 0.05) and significantly increased in DBT treatment groups compared with the model group (P < 0.05)
Summary
The World Health Organization estimates that the number of people in the world with diabetes has increased dramatically over recent years and is expected to reach 300 million by the year 2025 [1]. Diabetic complications are recognized as the most common cause of morbidity and mortality in diabetic patients. Diabetic nephropathy (DN), a serious and major complication of diabetes, has become the most common cause of end-stage renal disease (ESRD) in recent years. The first manifestation of diabetic nephropathy in humans is increased urinary albumin excretion, which usually progresses to nephroticrange proteinuria [2]. Increased urinary albumin (proteinuria) is a key component of this disease. Its development led to ESRD with increased morbidity and mortality for diabetic patients versus nondiabetic patients
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