Protective effects of Chrysanthemi Flos extract against streptozotocin-induced oxidative damage in diabetic mice

Abstract

The present study was aimed to evaluate the protective role of Chrysanthemi Flos extract (CFE) against free radical-mediated oxidative damage in liver and kidney of streptozotocin (STZ) - induced diabetic mice. Chrysanthemi Flos was extracted with methanol. Male ICR mice (n = 24) were divided into four groups. Diabetes was induced by STZ injection (ip) at a dose of 100 mg/kg. Animals of treated groups were given CFE and gliclazide (1000 and 50 mg kg −1 body weight) for 30 days. The results revealed that oral administration of CFE and gliclazide inhibited the increase in lipid peroxidation, aspartate transaminase (AST), alanin transaminase (ALT) and alkaline phosphatase (ALP) activities caused by STZ. Besides, a significant amelioration in antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) was observed. On the other hand, non enzymic antioxidant such as reduced glutathione (GSH) and ascorbic acid level was significantly increased while α-tocopherol level was decreased. Furthermore, CFE caused significant restoration in DNA fragmentation induced by STZ. These findings suggest that CFE has protective effects against STZ induced oxidative damage in liver and kidney of diabetic mice.