Protective Effects of Catechins Isolate From GMB4 Clone Green Tea Against EPC In Type 2 Diabetes Mellitus

Abstract

Introduction : This study aimed to evaluate the role of catechins isolates from GMB4 clone green to in the dynamics of endothelial progenitor cells (EPC) in type 2 diabetes mellitus (DM).Methods: 25 Wistar rats, were divided into 5 groups, including control group, type 2 diabetes mellitus group, treated daily administration of extracts of catechins isolate from GMB4 clones green tea at 20; 40; and 60 mg /kgBB doses for 6 weeks. EPCs analysis was performed by flow cytometry,levels of NO was performed using a spectrophotometer, serum levels of SDF-1a was performed with ELISA technique. Analysis of the expression of SDF-1 and CXCR-4 was performed using immunohistochemistry techniques.Results : The CD34+ and CD133+ count is lower in the DM group compared to the control group (P < 0.05). Of the three doses of catechins, only the highest dose were able to significantly increase CD34+ count compared to rats in the diabetes mellitus group (P < 0.05), Serum NO level is significantly higher in the DM the control group (P < 0.05). All three doses of catechins were able to significantly increase the expression of SDF-1a and CXCR4 in the aorta compared to the DM group or the control group (P < 0.05).Discussion : It can be concluded that high dose cathecin isolate from GMB-4 clone green tea (60 mg/kgBB) may trigger the proliferation and maturation of EPCs in rats with type 2 DM in environment with high level of NO, involving the interacton between SDF-1a and CXCR4 in the aorta.