Abstract
Chronic renal failure (CRF) is a serious disease related to increasing incidence and prevalence as well as decline in quality of life. Bu-Shen-Huo-Xue formula (BSHX), one of traditional herbal formulations, has been clinically employed to treat CRF for decades, but the mechanisms involved have not been investigated. In the present study, we investigated the effects of BSHX on some closely related parameters in 5/6 nephrectomy CRF rats. Rats with CRF were divided into five groups, namely, one control group, one enalapril group, and three BSHX treatment groups (0.25, 0.5, and 1 g/kg·d). The rats subjected to sham operation were used as a normal control. After eight weeks of treatment, BSHX significantly decreased the levels of Scr and BUN, downregulated the mRNA expression levels of TGF-β 1, CTGF, NF-κB, TNF-α, and OPN, upregulated the mRNA expression of PPARγ, and reduced in situ expression of fibronectin and laminins. Histological findings also showed significant amelioration of the damaged renal tissue. BSHX protects 5/6 nephrectomy rats against chronic renal failure probably via regulating the expression of TNF-α, NF-κB, TGF-β 1, CTGF, PPARγ, OPN, fibronectin, and laminins and is useful for therapy of CRF.
Highlights
The progression of chronic renal failure (CRF) is characterized by the development of glomerular and tubular lesions in which multiple factors can be involved [1]
We observed the effects of Bu-Shen-Huo-Xue formula (BSHX) on several growth factors and cytokines in 5/6 nephrectomy rats with CRF, including transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), tumor necrosis factor-α (TNF-α), nuclear factor kappa B (NF-κB), peroxisome proliferator-activated receptor
blood urea nitrogen (BUN) and serum creatinine (Scr) levels were decreased by eight weeks of BSHX treatment (0.25, 0.5, and 1.0 g/kg) in comparison with the control group
Summary
The progression of chronic renal failure (CRF) is characterized by the development of glomerular and tubular lesions in which multiple factors can be involved [1]. In China, Korea, and Japan, extensive experience and abundant clinical data in the treatment of CRF have been documented for traditional Chinese medicine, which has been developed over thousands of years [3, 4]. (Rhei radix et rhizoma), Vaccaria segetalis (Neck.) Garcke. BSHX has been clinically used to treat CRF in China, which possesses the efficacies of tonifying the kidney and nourishing qi and promoting blood circulation to remove blockage of channels. BSHX significantly decreases the levels of serum creatinine (Scr) and blood urea nitrogen (BUN) and delays CRF progression [5, 6]. We observed the effects of BSHX on several growth factors and cytokines in 5/6 nephrectomy rats with CRF, including transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), tumor necrosis factor-α (TNF-α), nuclear factor kappa B (NF-κB), peroxisome proliferator-activated receptor
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