Abstract
Ziziphus jujuba (jujube) seeds were extracted with 60% (v/v) aqueous ethanol assisted by homogenization and sonication followed by fractionation into n-hexane, chloroform, ethyl acetate, n-butanol, and water. The ethyl acetate fraction exhibited the highest levels of total phenolics (102.05 mg gallic acid equivalents/g fraction), total flavonoids (35.43 mg catechin equivalents/g fraction), and antioxidant capacity (271.67 and 66.02 mg vitamin C equivalents/g fraction in respective ABTS and DPPH assays) among the fractions tested. Ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis of the ethyl acetate fraction revealed 12 phenolic compounds: 3,4-dihydroxybenzoic acid, methyldopa, p-coumaric acid, dihydro-p-coumaric acid, rutin, isoquercetin, kaempferol 3-O-rutinoside, 3-O-cis-p-coumaroylalphitolic acid, 2-O-cis-p-coumaroylalphitolic acid, 3-O-trans-p-coumaroylalphitolic acid, 3-O-cis-p-coumaroylmaslinic acid, and 3-O-trans-p-coumaroylmaslinic acid. Phenolic compounds generally increased viability, reduced lactate dehydrogenase release, and attenuated intracellular oxidative stress in PC-12 cells. The results of this study suggest that Z. jujuba seeds may serve as an effective source of antioxidative agents to reduce oxidative stress and enhance neuronal cell viability.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
