Protective effects of berberine in chronic copper-induced liver and gill injury in freshwater grouper (Acrossocheilus fasciatus)

Abstract

This experiment aimed to investigate the protective effects of berberine on copper-induced liver and gill toxicities in freshwater grouper (Acrossocheilus fasciatus). Fish (initial weight 1.56 ± 0.10 g) were randomly distributed into 12 tanks (80 L, 20 fish per tank) and divided into four experimental groups: The control group, exposed to 0.02 mg/L Cu2+ (Cu group), exposed to 0.02 mg/L Cu2+ and fed 100 mg/kg berberine (BBR100 group), and exposed to 0.02 mg/L Cu2+ and fed 400 mg/kg berberine (BBR400 group). After a 30-day experiment, the results showed that berberine significantly increased the activities of catalase and glutathione peroxidase in the liver, gills, and serum inhibited by Cu2+ exposure (P < 0.05). Berberine inclusion significantly decreased the activities of lysozyme and acid phosphatase, as well as the content of immunoglobulin M compared to the Cu group (P < 0.05). Berberine significantly suppressed the expression of the proinflammatory cytokines interleukin-1β, interleukin-6 signaling transducer, and NLR family pyrin domain containing 3 in the liver and gills induced by Cu2+ exposure while downregulating the expression of the anti-inflammatory cytokine transforming growth factor β1. Additionally, berberine significantly reduced the activities of the liver injury markers alanine transaminase and aspartate transaminase, the levels of total cholesterol and triglyceride in serum, as well as alleviated the histopathological damage in the liver and gills caused by Cu2+ exposure. In summary, berberine enhanced antioxidant capacity, mitigated inflammation, and exerted significant protective effects on liver and gill damage in freshwater grouper under Cu2+ exposure.