Protective effects of Baicalin on intestinal mucosa injury by inhibiting nuclear factor-κB expression in rats with partial common bile duct ligation

Abstract

Objective To investigate the effects of Baicalin on intestinal mucosal injury in rats with partial common bile duct ligation (PCBDL). Methods Forty male Wistar rats were randomly divided into 4 groups equally: sham operation, PCBDL, PCBDL1 and PCBDL2.Rats in PCBDL, PCBDL1 and PCBDL2 groups were subjected to partial common bile duct ligation.Baicalin [80 mg/(kg·d)]was fed in PCBDL1 group (for 2 weeks) and PCBDL2 group(for 3 weeks), while for other groups, 9 g/L saline in the same volume was fed.Ileum mucosa were prepared for microscopic examination.The intestinal mucosal injury in rats was observed and scored.The level of NF-κB mRNA expression by Fluorescent in Situ Hybridization, and the level of NF-κB protein were determined by immunohistochemistry. Results 1.Compared with PCBDL group(3.2±0.5), the pathological severity scores of intestinal mucosa significantly declined (F=21.120, P<0.01) in PCBDL1 group (1.9±0.2) and PCBDL2 group (1.5±0.3).2.Compared with sham ope-ration group(0.066±0.006), PCBDL1 group(0.107±0.011), and PCBDL2 group(0.098±0.010), NF-κB expression in PCBDL group(0.155±0.012)presented significantly up-regulation(F=76.8, P<0.01).3.Compared with sham operation, PCBDL1 group, and PCBDL2 group, the positive expression rates of NF-κB mRNA of intestinal mucosal epithelium in PCBDL group significantly increased. Conclusions It is suggested that Baicalin exert protective effects on the intestinal mucosal injury in rats with PCBDL, partially by inhibiting NF-κB mRNA, down-regulating NF-κB protein expression of intestinal mucosal epithelial cells. Key words: Partial common bile duct ligation; Baicalin; Intestinal mucosal epithelial cells; Nuclear factor-κB; Nuclear factor-κB mRNA