Protective effects of astragalosides on dexamethasone and Aβ25–35 induced learning and memory impairments due to decrease amyloid precursor protein expression in 12-month male rats

Abstract

Alzheimer’s disease (AD) is a chronic neurodegenerative disorder of the elderly characterized by learning and memory impairment. Stress level glucocorticoids (GCs) and β-amyloid (Aβ) peptide deposition are found to be correlated with dementia progression in patients with AD. The astragalosides (AST) was extracted from traditional Chinese herb Astragalus membranaceous. In this study, 12months male rats were treated with Aβ25–35 (10μg/rat, hippocampal CA1 injection) and dexamethasone (DEX, 1.5mg/kg, ig) and AST (8, 16 and 32mg/kg, ig) or ginsenoside Rg1 (Rg1, 5mg/kg, ig) for 14days. We investigated the protective effect of AST against DEX+Aβ25–35 injury in rats and its mechanisms of action. Our results indicate that DEX+Aβ25–35 can induce learning and memory impairments and increase APP and Aβ1–40 expression. AST (16, 32mg/kg) or Rg1 (5mg/kg) treatment significantly improve learning and memory, down-regulate the mRNA levels of APP and β-secretase, decrease expression of APP and Aβ1–40 in hippocampus. The results indicated that DEX might increase hippocampal vulnerability to Aβ25–35 and highlight the potential neuronal protection of AST.