Abstract
Adipose-derived mesenchymal stem cells (ADSCs) are multipotent stem cells that promote therapeutic effects and are frequently used in autologous applications. Little is known about how ADSCs respond to genotoxic stress and whether or not donor age affects DNA damage and repair. In this study, we used the comet assay to assess DNA damage and repair in human ADSCs derived from young (20-40 years), middle-aged (41-60 years), and older (61+ years) donors following treatment with H2O2 or UV light. Tail lengths in H2O2-treated ADSCs were substantially higher than the tail lengths in UV-treated ADSCs. After 30 minutes of treatment with H2O2, ADSCs preconditioned with alpha phenyl-tert-butyl nitrone (PBN) or ascorbic acid (AA) showed a significant reduction in % tail DNA. The majority of ADSCs treated with PBN or AA displayed low olive tail movements at various timepoints. In general and indicative of DNA repair, % tail length and % tail DNA peaked at 30 minutes and then decreased to near-control levels at the 2 hour and 4 hour timepoints. Differently aged ADSCs displayed comparable levels of DNA damage in the majority of these experiments, suggesting that the age of the donor does not affect the DNA damage response in cultured ADSCs.
Highlights
Adipose-derived mesenchymal stem cells (ADSCs) are multipotent cells that can differentiate into adipocytes, chondrocytes, and osteoblasts [1]
Tail length is defined as the distance of DNA migration from the nuclear body of Representative slides of DNA comets from human Adipose‐derived mesenchymal stem cells (ADSCs) treated with H2O2 for 30 minutes are shown
In response to UV light, ADSCs from younger donors displayed the longest tail length at the initial DNA damage timepoint. This difference was not statistically significant, and the measured tail lengths at both the initial DNA damage and end DNA damage timepoints were comparable for ADSCs from differently aged donors. At both the initial DNA damage and end DNA damage timepoints, the tail length in ADSCs treated with UV light was drastically smaller than the tail length in ADSCs treated with H2O2 (Fig. 2)
Summary
Adipose-derived mesenchymal stem cells (ADSCs) are multipotent cells that can differentiate into adipocytes, chondrocytes, and osteoblasts [1]. They have been reported to differentiate into other cell types, including skeletal and cardiac muscle cells [1, 2]. ADSCs represent an attractive therapeutic source of adult stem cells and have been the focus of many preclinical and clinical studies geared towards a variety of applications [8, 9]. ADSC-centered therapies have been shown to be promising for a variety of clinical applications, including neurodegenerative disorders [10], arthritis [11], wound www.aging‐us.com healing [12], diabetes [13, 14], autoimmune disease [4, 14], plastic surgery [15], and many others
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