Protective effects of alkaloid compounds from Nelumbinis Plumula on tert-butyl hydroperoxide-induced oxidative stress.

Yong Xie,Shao-Xiao Zeng,Yi Zhang,Bao-Dong Zheng,Ze-Bin Guo,Long-Tao Zhang

doi:10.3390/molecules180910285

Abstract

This study was conducted to investigate the effect of Nelumbinis Plumula total alkaloid (NPA) and its main alkaloid components on oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in the human hepatocellular HepG2 cell line. According to HPLC analysis, several major alkaloid compounds such as liensinine, isoliensinine and neferine were present in NPA. The cytotoxic effects in 0.55 mM t-BHP-induced HepG2 cells were significantly inhibited by NPA and the major compound in NPA, neferine, showed the strongest activities. The protective effect of neferine against oxidative stress induced by t-BHP may be associated with decreased ROS formation, TBARS generation, LDH release and increased GSH levels, suggesting their involvement of the cytoprotective on oxidative stress. The effects were comparable with quercetin, which was used as positive control. Overall, total alkaloid and alkaloid compounds from Nelumbinis Plumula displayed a significant cytoprotective effect against oxidative stress. Further study is needed to elucidate the relationship between the chemical structures of the components in NPA and their protective effect on oxidative stress.

Highlights

Clinical and experimental studies suggest that excessive reactive oxygen species (ROS) may bring about oxidative stress and attack cellular biomolecules such as lipids, proteins and DNA [1]
The total alkaloid content (TAC) of Nelumbinis Plumula total alkaloid (NPA) was determined by an acid dye complexing colorimetric method
DPPH is a quite stable free radical that shows a maximum absorption at 516 nm, which may be reduced by adding some antioxidant into the system [15], so we can determine the antioxidant activity potency by measuring the DPPH radical-scavenging activity

Summary

Introduction

Clinical and experimental studies suggest that excessive reactive oxygen species (ROS) may bring about oxidative stress and attack cellular biomolecules such as lipids, proteins and DNA [1]. The oxidative stress-induced damage can disrupt cellular function and membrane integrity, thereby leading to cell death [2]. Natural antioxidants may play an important role in lengthening the shelf life of cellular biomolecules such as DNA, proteins and lipids, reducing cancer or death of cells and harmful substances by regulating oxidative stress [4]. Lotus seeds have been widely consumed by people in Asia. Different parts of the lotus such as germs, leaves and rhizomes have been reported for their chemical compounds and biological activities

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