Protective effects of 3,4',5,7‐tetrahydroxyflavone against squalene monohydroperoxide‐induced skin wrinkles and its green extraction using deep eutectic solvents

Abstract

AbstractThree flavone derivatives, 3,4′,5,7‐tetrahydroxyflavone (3,4′,5,7‐THF) and their glycosides 3,4′,5,7‐tetrahydroxyflavone‐3‐rhamnoglucoside, and 3,4′,5,7‐tetrahydroxyflavone‐3‐glucoside, were identified by partial enzymatic conversion of Camellia sinensis seed extract (CSSE). 2,2‐Diphenyl‐1‐picrylhydrazyl radical scavenging activity of these compounds and lipid peroxidation inhibition effects of 3.4′,5,7‐THF were tested to evaluate their antioxidant properties. To investigate the effects of 3,4′,5,7‐THF on squalene monohydroperoxide (SQLOOH)‐induced wrinkle formation, the dorsal skin of SKH1 hairless mice was used. Four weeks of treatment with 3,4′,5,7‐THF reduced wrinkle formation, in contrast to that observed in the group treated with SQLOOH only. A green and simple method has been suggested for the extraction of 3,4′,5,7‐THF from CSSE hydrolysate with nine deep eutectic solvents. A mixture of choline chloride and xylose yielded the best extraction result. These findings suggest that 3,4′,5,7‐THF is a natural compound with potential application in preventing SQLOOH associated skin aging.