Protective Effects Induced by Two Polyphenolic Liquid Complexes from Olive (Olea europaea, mainly Cultivar Coratina) Pressing Juice in Rat Isolated Tissues Challenged with LPS.

Lucia Recinella,Carla Ghelardini,Sheila Leone,Luigi Brunetti,Claudio Ferrante,Lorenzo Di Cesare Mannelli,Giustino Orlando,Annalisa Chiavaroli,Luigi Menghini

doi:10.3390/molecules24163002

Lucia Recinella, Carla Ghelardini + Show 7 more

Open Access

https://doi.org/10.3390/molecules24163002

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Abstract

MOMAST(®) HY100 and MOMAST(®) HP30 are polyphenolic liquid complexes from olive pressing juice with a total polyphenolic content of 100 g/kg (at least 50% as hydroxytyrosol) and 36 g/kg (at least 30% as hydroxytyrosol), respectively. We investigated the potential protective role of MOMAST(®) HY100 and MOMAST(®) HP30 on isolated rat colon, liver, heart, and prefrontal cortex specimens treated with Escherichia coli lipopolysaccharide (LPS), a validated ex vivo model of inflammation, by measuring the production of prostaglandin (PG)E2, 8-iso-PGF2α, lactate dehydrogenase (LDH), as well as cyclooxygenase (COX)-2, tumor necrosis factor α (TNFα), and inducible nitric oxide synthase (iNOS) mRNA levels. MOMAST(®) HY100 decreased LPS-stimulated PGE2 and LDH levels in all tested tissues. Following treatment with MOMAST(®) HY100, we found a significant reduction in iNOS levels in prefrontal cortex and heart specimens, COX-2 and TNFα mRNA levels in heart specimens, and 8-iso-PGF2α levels in liver specimens. On the other hand, MOMAST(®) HP30 was found to blunt COX-2, TNFα, and iNOS mRNA levels, as well as 8-iso-PGF2α in cortex, liver, and colon specimens. MOMAST(®) HP30 was also found to decrease PGE2 levels in liver specimens, while it decreased iNOS mRNA, LDH, and 8-iso-PGF2α levels in heart specimens. Both MOMAST(®) HY100 and MOMAST(®) HP30 exhibited protective effects on multiple inflammatory and oxidative stress pathways.

Highlights

It has been well established that olive tree (Olea europaea) polyphenols have healthy beneficial effects, including the prevention of several chronic diseases, such as cancer and aging-associated degenerative diseases [1,2]
Considering these findings, we performed a second set of experiments aimed to evaluate the modulatory effects of MOMAST(®) HY100 (10, 50, and 100 μg/mL) and MOMAST(®) HP30 (22, 110, and 220 μg/mL) supplementation on oxidative stress and multiple inflammatory pathways in colon, liver, heart, and prefrontal cortex specimens challenged with LPS
The beneficial effects of plant polyphenols in humans have been confirmed by a large body of evidence [15,16,17,18]

Summary

Introduction

It has been well established that olive tree (Olea europaea) polyphenols have healthy beneficial effects, including the prevention of several chronic diseases, such as cancer and aging-associated degenerative diseases [1,2]. These beneficial properties could be mainly related to the antioxidant activity of olive tree polyphenols, which were found able to both scavenge free radicals and reactive oxygen species and activate endogenous antioxidant enzymes, including glutathione peroxidase, glutathione reductase, and glutathione S-transferase [3,4,5]. MOMAST(®) HY100 and MOMAST(®) HP30 (Bioenutra, Ginosa, TA, Italy) are polyphenolic liquid complexes from olive (Olea europaea, mainly Cultivar Coratina) pressing juice with a total polyphenolic content of 100 g/kg (at least 50% as HT) and 36 g/kg (at least 30% as HT), respectively (Tables 1 and 2)

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