Protective Effect on Oxidative Stress Injury of Xiongma Dripping Pills Containing Serum on PC12 Cells

Abstract

Object: The antioxidant dose-effect relationship and the mechanism of Xiongma Dripping Pills were study to explore the protective effects of oxidative damage in PC12 cells of different doses of Xiongma Dripping Pills containing serum. Method: Oxidative stress injury model of PC12 cells was established by peroxide hydrogen (H2O2), sodium nitroprusside (SNP), content and cell culture supernatant lactate dehydrogenase (LDH) and the liquid content of glutathione peroxidase (GSH-PX) were detected by chemical colorimetric determination. Results: There was a plasma concentration peaked in Xiongma Dripping Pills 8 times (6g•kg-1) dose group, ferulic acid plasma concentration was 10.59 ± 2.92, the plasma concentration gastrodin 18.13 ± 4.63. H2O2 and SNP could cause injury in PC12 cells, and increased LDH leakage, reduce the content of GSH-PX (P<0.01). Gung Ma the Dripping Pills can reduce LDH leakage, increase the content of GSH-PX. Xiongma Dripping Pills 8-fold dose group content is the most significant.Conclusion: There was a significant protective effect on Xiongma Dripping Pills serum containing H2O2 and SNP induced oxidative damage of PC12 cells and its mechanism was relevant with clearing the free radicals, enhanceing antioxidant enzyme activity in vivo. There was the most significant effect on Xiongma Dripping Pills 8-fold dose group, and there was positive correlation on plasma concentration and pharmacological effects.