Protective effect of Xiongbitongcapsule on liver injury in hyperlipemic rats

Abstract

Objective To investigate the protective role of Xiongbitong capsule against liver injury in hyperlipemic rats.Methods Sixty Wistar male rats were randomly divided into 5 groups（12 rats in each group）： a blank group, a model group, a simvastatin group（10 mg/kg, 2 ml intragastric administration daily）, a Xiongbitong capsule high-dose group（25 mg/kg, 2 ml intragastric administration daily）, and a Xiongbitong capsule low-dose group（12.5 mg/kg, 2 ml intragastric administration daily）. Hyperlipidemia model in rats was indeuced by hyperlipidemic diet. The simvastatin group was intragastric administrated with simvastatin suspension 2 ml（10 mg/kg daily）, and the rats in the control group and the model group were intragastric administrated with equal volume of saline. After 10 weeks, the serum leves of total cholesterol（TC）, triacylglycerol（TG）, low-density lipoprotein cholesterol（LDL-C）, high-density lipoprotein cholesterol （HDL-C）, nitric oxide（NO）, endothelin1（ET-1）, and the whole blood viscosities（high-, medium-, low-shear）were measured. Liver injury were evaluated with histopathologic examination by H.E. staining. The expressions of intercellular adhesion molecule-1（ICAM-1）, monocyte chemoattractant protein-1（MCP-1）in hepatic tissue were measured by immunohistochemical staining.Results The serum leves of TC（1.47± 0.10 mmol/Lvs. 3.48±0.19 mmol/L）, TG（0.38±0.11 mmol/Lvs. 0.95±0.14 mmol/L）, LDL-C（1.48± 0.18 mmol/Lvs. 2.39±0.22 mmol/L）, ET-1（145.81±18.65 pg/mlvs. 177.70±17.70 pg/ml） in the Xiongbitong capsule high-dose group were significantly lower than those in the model group（allP〈0.01）, HDL-C（1.21±0.14 mmol/Lvs. 0.65±0.10 mmol/L）and NO（31.28±2.36μmol/Lvs. 19.61±1.28μmol/L） significantly lower than those in the model group（allP〈0.01）, the expressions of ICAM-1（0.133±0.019vs. 0.187±0.011）and MCP-1（0.153±0.014vs. 0.264±0.020）significantly lower than those in the model group（allP〈0.01）. The liver injury in the Xiongbitong capsule high-dose group decreased than that in the model group. Conclusions Xiongbitong capsule can protect against liver injury via regulating lipid metabolism, protecting endothelial function and down regulating expressions of MCP-1 and ICAM-1. Key words: Xiongbitong capsule; Hyperlipidemias; Endothelial cells; Nitric oxide; Monocyte chemoattractant proteins