Abstract
The protective effect of tyrosol on apoptosis in PC12 cell induced by paraquat (PQ) was studied. The PC12 cell was cultured in RPMI-1640 medium supplemented with 10% newborn calf serum at 37°C in a 5% CO2 incubator. 800 μm of PQ solution was added to establish an oxidative-stress injury model in vitro. The protective effect of tyrosol was evaluated by using this model. We compared the lactate dehydrogenase (LDH) release rate and malondialdehyde (MDA) content of PC12 cells in different treatment group. The cell apoptosis was detected by using flow cytometry (FCM) and the cell proliferation by using 5-diphenyltetrazolium (MTT) assay. The results showed that pretreatment with tyrosol could improve cell growth and proliferation in a concentration dependent manner, reduce the release of LDH and MDA content, suppress the PQ-induced apoptosis of PC12 cells. Therefore, these results suggested that tyrosol possessed protective activity of PC12 cell’s injury by PQ in vitro. Key words: Tyrosol, PC12 cell, paraquat, protective effect.
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