Abstract
BackgroundTo investigate the protective effect of Ixeris Sonchifolia (Bae.) Hance (ISH) extract on herpes simplex virus keratitis (HSK) in mice.MethodsA mouse model of HSK was established by inoculating 60 mice (60 right eyes) with herpes simplex virus type 1 (HSV-1) by corneal scratch. The other 15 mice as blank control only received corneal scratch but without HSV-1. From the 2nd day after the successful modeling, the experimental group was fed with ISH total flavonoids (50, 100 and 200 mg/kg) orally, twice a day for 14 days. The model group and control group were given the same amount of normal saline. The pathological changes of cornea were observed once a day by slit lamp microscopy combined with fluorescein staining. The corneal histopathological examination, the survival status and the serum levels of interleukin-2 (IL-2), IL-4 and interferon-gama (INF-γ) were performed at the end of the experiment.ResultsThe result showed that ISH could significantly improve the corneal lesion degree, increase mice survival rate, and markedly increase the levels of IL-2 and INF-γ, reduce the levels of IL-4 in serum of mice.ConclusionsISH could increase the anti-virus ability, promote the healing of corneal inflammation and alleviate the pathological damage of cornea, which suggested that ISH has a potential and valuable therapeutic effect on the HSK.
Highlights
To investigate the protective effect of Ixeris Sonchifolia (Bae.) Hance (ISH) extract on herpes simplex virus keratitis (HSK) in mice
Herpes simplex keratitis (HSK) is a corneal infection caused by herpes simplex virus (HSV), it is a serious
herpes simplex virus type 1 (HSV-1) infect detection In order to detect if the animal model was successfully established, HEK293T normal cells were used to the virus infection detection object
Summary
Materials and reagents Mouse interferon-gama (INF-γ, Batch No 978941030), interleukin-2 (IL-2, Batch No 948941121) and interleukin-6 (IL-4, Batch No 948941123) were purchased from Shenzhen Jingmei Bioengineering Co., Ltd. (Shenzhen, China). 1% Sodium fluorescein eye drops obtained from the preparation room of Xuanwu Hospital, Capital Medical University (Beijing, China). 1% Sodium fluorescein eye drops obtained from the preparation room of Xuanwu Hospital, Capital Medical University (Beijing, China). Animals Seventy-five adult male BALB/c mice (body weight 20 ± 2 g) aged 6 weeks were provided by the animal experimental center of Capital Medical University (Beijing, China). All the mice were reared in SPF grade environment with the temperature and humidity of 24 ± 1 °C and 50% ± 5% under the 12 h day/night cycle in the animal experimental center of Capital Medical University. Containing the virus with TCID50 of 10–9.31/0.l mL onto the surface of the right eye cornea, and massaged the eyelids of the mice gently for 2 min. While for the control group mice, dripped 5 μL of DMEM without virus onto the surface of the right eye cornea, and massaged the eyelids of the mice gently for 2 min. P < 0.05 and < 0.01 were regarded as statistically significant
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
