Abstract
Objective: The protective effect and possible mechanism of total flavones of buckwheat flowers (TFBF) onexperimental hepatic impairment in mice were studied. Methods: The hepatic impairment model of mice was inducedby injecting carbon tetrachloride (CCl4) subcutaneously (sc) every 4 days for 7 times. Meanwhile, mice in the twotreatment groups were given TFBF at dosages of 0.04 g•kg-1•d-1 and 0.02 g•kg-1•d-1 respectively through intragastric (ig)injection, and mice in the positive control group were treated with methionine (MET) by contrast. Next the day CCl4was lastly injected, half the mice were killed. The contents of alanine aminotransferase (ALT) in serum and ALT,superoxidase dismutase (SOD), glutathione (GSH), malonaldehyde (MDA), triglyceride (TG), total cholesterol (TC) inliver tissue, the liver indexes (LI), and the hepato-pathologic changes of the mice were examined. The rest mice weregiven identical treatment for another 2 weeks. Results: TFBF could inhibit the rising of serum ALT, liver MDA, TG, TC,LI, and the lowering of liver SOD and GSH in CCl4-induced hepatic impairment mice. It could obviously ease thehepato-pathologic damages as well. Conclusion: TFBF could effectively protect the hepatic impairment in CCl4-inducedmice.
Highlights
The research is financed by Science Committee of Hebei Province
3.1 Influence of total flavones of buckwheat flowers (TFBF) on weight and liver indexes in CCl4-induced mice From table 1, we can see that the mice in model group lost some weight instead of putting on when treated with CCl4.This meant that CCl4 could inhibit the mice from growing
These free radicals combine covalently to the macromolecules in hepatic cells, which produces lipid peroxidants, damages the constructions and functions of the cell membranes, and dysfunctions the membrane transportation and calcium reserves of the cells, and results in the death of the cells (Nordmann, 1992,29-34). The results of this experiment showed that CCl4 could obviously increase the amount of serum ALT and liver MDA, TG and total cholesterol (TC), but decrease that of liver superoxidase dismutase (SOD) and GSH in mice
Summary
The research is financed by Science Committee of Hebei Province. No 03276421 (Sponsoring information) Abstract Objective: The protective effect and possible mechanism of total flavones of buckwheat flowers (TFBF) on experimental hepatic impairment in mice were studied. 1.1 Animal Kunming strain mice of either sex, Grade Ċ, weighing (21±2) g each, were provided by experimental animal centre of Henan Province, China. 1.3 Instruments Rotating evaporator (Tianjin); Boehringer Mannheim / Hitachi 7150 Analyzer (Japan); RILI 850 fluorescence emission spectrophotometry equipment (Japan); 721 spectrophotometer (Shanghai); LXJ-II centrifugal equipment (Shanghai); MP200A electronic balance (Shanghai); OLYMPUS VANOXPM-10AD microscopy (Japan)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
