Protective effect of Thymol essential oil from Thynus quinquecostatus extracts on UV irradiation‐induced skin aging by supressiong MMP‐1 expression and collagen degradation

Abstract

Solar ultraviolet (UV) radiation is a main extrinsic factor for skin aging, which causes impairment of skin integrity through the induction of matrix metalloproteinases (MMPs), such as MMP‐1. Thynus quinquecostatus extracts are considered as potent anti‐photoaging agents due to including various active ingredients. To identify anti‐photoaging agents, in the present study, we examined preventing effect of thymol essential oil from Thynus quinquecostatus extracts on UV‐induced skin photo‐damage. First, we determined that the treatment concentration of without cytotoxicity on thymol by MTT assay in HaCaT cells. Next, we examined the attenuation of antiaging activity that the determined of MMP‐1 gene and protein expression levels in UVB‐induced keratinocyte. As a result, it was confirmed that the thymol and Thynus quinquecostatus extracts dramatically decreased UVB‐induced expression of MMP‐1 in a dose‐dependent manner. Our data showed that thymol and Thynus quinquecostatus extracts significantly attenuated UV‐induced phosphorylation of mitogen‐activated protein kinase (MAPK) signaling components ERK1/2, JNK1/2, and p38 proteins. This result suggests that the ERK1/2 kinase pathways likely contribute to the inhibitory effects of thymol and Thynus quinquecostatus extracts on UV‐induced MMP‐1 production in human keratinocytes. Taken together, our findings suggest that tymole essential oil from Thynus quinquecostatus extracts is a protective agent for skin anti‐photoaging through the prevention of UV‐induced MMP‐1 production.Support or Funding InformationFunding from the Region Industry Total Information Service Program (R0004665), Republic of Korea, supported this work.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.