Protective effect of three diallyl sulphides against glucose-induced erythrocyte and platelet oxidation, and ADP-induced platelet aggregation

Abstract

Isolated human erythrocyte membrane and platelet were used to study the antioxidative and anti-aggregative effects of three diallyl sulphides (diallyl sulphide, DAS; diallyl disulphide, DADS; diallyl trisulphide, DAT) against glucose-induced oxidation and adenosine 5′-diphosphate (ADP)-induced platelet aggregation. Three sulphide agents showed dose-dependent antioxidative protection against glucose-induced erythrocyte membrane oxidation ( p<.05), and these agents at 10 μM significantly increased the retention of α-tocopherol in erythrocyte membrane ( p<.05), in which DAT was the most effective agent ( p<.05). Three sulphide agents significantly inhibited 30 and 50 mM of glucose-induced platelet oxidation ( p<.05). The anti-aggregative activity of each sulphide agent was dose dependent ( p<.05), in which DAT showed the greatest inhibitory effect on platelet aggregation than DADS, followed by DAS ( p<.05). After ADP stimulation, the malondialdehyde (MDA) formation in platelets treated with sulphide agents was significantly less ( p<.05), in which DADS and DAT showed similar antioxidative activities ( p>.05). These results suggested that DADS and DAT could be considered as strong antioxidative and antithrombotic agents to prevent or control oxidative damage and platelet hyperactivity.