Protective effect of the total flavonoids of drynariae on renal reperfusion injury after hemorrhagic shock

Abstract

Objective To investigate the protective effect of total flavonoids of drynaria on hemorrhagic shock reperfusion renal injury. Methods The 30 Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, experimental group (hemorrhagic shock group) and drynaria fortunei group, 10 in each group. In drynaria fortunei group and experiment group, the blood from the femoral artery was withdrawn until the mean arterial pressure reached 40 mmHg in 90 min, and then the withdrawn blood transfusion were reinfused and blood pressure recovered to the base value of 90%. The rats of drynaria fortunei group were given intraperitoneal injection of Drynaria total flavonoid 300 mg/kg, and experimental group and sham operation group were injected with 3 ml physiological saline. Blood creatinine (SCr) levels, the renal cell apoptosis index, the expression of Bcl-2 and mRNA Bax in kidney were detected. Results Compared with the experimental group, serum SCr (78.36 ± 5.31 mmol/L vs. 151.43 ± 11.8 mmol/L) of drynaria fortunei group decreased significantly (P<0.01); the renal cell apoptosis (19.56% ± 4.37% vs. 14.31% ± 2.83%), the expression level of Osteopractic rats of Bax group mRNA (1.24 ± 0.05 vs. 0.91 ± 0.07) of drynaria fortunei group decreased significantly; and the Bcl-2 mRNA (0.65 ± 0.05 vs. 1.38 ± 0.07) and the expression level and Bcl-2/bax ratio (0.52 ± 0.06 vs. 1.51 ± 0.08) of drynaria fortunei group increased significantly (P<0.01). Conclusions The drynaria total flavonoids on rats with hemorrhagic shock and resuscitation of renal injury has a protective effect, and its mechanism may improve the expression of Bcl-2 and mRNA, increase the ratio of bcl-2/bax, and inhibit or block the apoptosis of renal tissue. Key words: Kidney; Shock, hemorrhagic; Reperfusion injury; Drynaria total flavonoid; Apoptosis; Genes, bcl-2; Rats