Abstract
BackgroundVaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells.ObjectiveTo evaluate the effects of vaccination with mixed naked DNA plasmids encoding Der p 1, Der p 2, Der p 3, Der f 1, Der f 2, and Der f 3, the major house dust mite allergens on the allergic inflammation to the whole house dust mites (HDM) crude extract.MethodsThree hundred micrograms of these gene mixtures were injected into muscle of BALB/c mice. Control mice were injected with the pcDNA 3.1 blank vector. After 3 weeks, the mice were actively sensitized and inhaled with the whole house dust mite extract intranasally.ResultsThe vaccinated mice showed a significantly decreased synthesis of total and HDM-specific IgE compared with controls. Analysis of the cytokine profile of lymphocytes after challenge with HDM crude extract revealed that mRNA expression of interferon-γ was higher in the vaccinated mice than in the controls. Reduced infiltration of inflammatory cells and the prominent infiltration of CD8+ T cells were observed in histology of lung tissue from the vaccinated mice.ConclusionVaccination with DNA encoding the major house dust mite allergens provides a promising approach for treating allergic responses to whole house dust mite allergens.
Highlights
Vaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells
It has been reported that IgE-mediated inflammation to mites is associated with diseases such as asthma, allergic rhinitis, and atopic dermatitis and that the relatively minor determinants of house dust mite (HDM) allergens show IgE binding with 40% or more of allergic sera [1,2,3,4]
We investigated immune responses by gene vaccination with plasmid DNA encoding major HDM allergens (Der p 1,2, and 3, and Dermatophagoides farinae (Der f) 1,2, and 3) to challenges with whole HDM crude extract in sensitized mice
Summary
Vaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells. Clinical and Molecular Allergy 2006, 4:4 http://www.clinicalmolecularallergy.com/content/4/1/4 treating HDM-induced allergy. Vaccinations with naked DNA encoding antigen were reported to induce long-lasting cellular and humoral immune tolerance [6,7]. A recent literature search showed that injection of plasmid DNA encoding Der p 5 in rat inhibits Der p 5-specific IgE antibody production and an allergic response such as histamine release and airway hyperresponsiveness to Der p 5 [11]. We investigated immune responses by gene vaccination with plasmid DNA encoding major HDM allergens (Der p 1,2, and 3, and Der f 1,2, and 3) to challenges with whole HDM crude extract in sensitized mice
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
