Abstract
Objective: The main objective of the present study was to investigate the ameliorative potential of synthetic antioxidants mixture comprising N-acetyl cysteine, ascorbic acid, tocopheryl acetate, and thiamine as micronutrient combinational therapy and ethanolic extract of Bacopa monnieri as herbal antioxidant therapy approach against lead toxicity-induced metabolic dysfunctions in vitro.
 Methods: Experimental study involved in vitro exposure of mice brain and liver homogenates to different doses (100 μM, 250 μM, and 500 μM) of lead acetate. The study also involved coadministration of high-dose lead acetate (500 μM) and specific dosage of synthetic antioxidants or ethanolic extract of B. monnieri separately to homogenate cultures. Alterations in metabolic parameters of protein levels and lipid peroxidation were analyzed for evaluating the protective effect of synthetic antioxidants and B. monnieri against lead intoxication.
 Results: Results revealed dose-dependent statistically significant (p<0.001) reduction in protein levels and elevation in lipid peroxidation in lead acetate exposed mice brain and liver homogenates as compared to their respective control groups. Coadministration of lead acetate and synthetic antioxidants mixture or B. monnieri in the brain and liver homogenates conferred protection and manifested maintenance of studied biochemical parameters nearest to control groups. Ameliorative efficacy of B. monnieri against lead-induced neurotoxicity and hepatotoxicity was found to be more pronounced than that of a mixture of synthetic antioxidants.
 Conclusion: Synthetic antioxidants mixture (N-acetyl cysteine, ascorbic acid, tocopheryl acetate, and thiamine) and B. monnieri exhibited remarkable therapeutic efficacy against lead toxicity-induced metabolic dysfunctions in mice brain and liver homogenates by virtue of their antioxidant, neuroprotective, and hepatoprotective abilities.
Highlights
Lead metal is considered as one of the most pervasive and dreaded occupational and environmental toxicants of global concern
Results revealed that protein content statistically significantly (p
Results emphasized that administration of mixture of synthetic antioxidants as well as ethanolic extract of B. monnieri separately (Groups VII and VIII) along with the highest concentration (500 μM) of lead in brain homogenate cultures statistically very significantly exerted protective effect against lead toxicity (p
Summary
Lead metal is considered as one of the most pervasive and dreaded occupational and environmental toxicants of global concern. On the criteria of the occurrence frequency of toxicological consequences and human exposure potential, Agency for Toxic Substances and Disease Registry has placed lead metal at the second position in the priority list of “top 20 hazardous substances” [1]. Lead exposure has the propensity to induce alterations in mammalian metabolism due to its potent systemic toxicity. Lead exposure has been associated with triggering of detrimental effects on the nervous system [6,7,8], hemopoietic system [9], hepatic system [10,11], and reproductive system [12]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
