Abstract
Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.
Highlights
In humans, skin is the largest organ of the integumentary system
HFPS Inhibits Collagenase from Clostridium Histolyticum and Elastase from Porcine Pancreas. Both collagenase and elastase belong to proteases that break down proteins
Relative activities of both enzymes were decreased in the cells pretreated with HFPS in a dose-dependent manner. These results suggest that HFPS may act as an inhibitor of fibroblast collagenase and elastase and may prevent wrinkle formation induced by UVB irradiation
Summary
Skin is the largest organ of the integumentary system. It undergoes chronological aging similar to other organs. Skin is in direct exposure to the outside environment and it undergoes aging as a consequence of environmental damage [1]. Ultraviolet (UV) irradiation from sunlight is the primary environmental factor that induces human skin aging and results in pigment accumulation and wrinkle formation. Human skin is frequently affected by oxidative stress caused by continuous exposure to UV irradiation from sunlight. Skin exposed to UV and environmental oxidizing pollutants is associated with diverse abnormal reactions including inflammatory responses, epidermal hyperplasia, the breakdown of collagen, and melanin accumulation [2,3]
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