Abstract
Objective: to reveal the effects of sea buckthorn seed oil on acetaminophen induced variations (Diameter of hepatocytes and vacuolization) in liver of rats.
 Methods: it was experimental study, conducted at Post Graduate Medical Institute. This study was performed on 24 adult rats, which were then randomly separated into 4 groups A,B,C and D. the experiment lasted for 13 days. Group A was control received normal saline and distilled water. Group B, received acetaminophen750mg/kg as single dose on day 10. Group C and D received acetaminophen at dose of 750mg/kg along with sea buckthorn seed oil extract 2.6mg/kg and 5.2mg/kg. Liver was removed and fixed in 10% formalin. To observe the effect of acetaminophen and sea buckthorn seed oil , slides were prepared for histological examination. The diameter of hepatocytes and vacuolization was observed. The evaluation of results was done by using SPSS 21.
 Results: In Group B, vacuolization was seen in all animals and significant increase in mean diameter (11.9± 1.5 μm) of hepatocytes was seen. However in Group C, vacuolization was absent in all animals and mean hepatocyte diameter was (6 ± 0.6 μm). In Group D, no vacuolization was seen in all animals and mean hepatocyte diameter decreased to (5.2± 1.5 μm) after administration of SBT seed oil.
 Conclusion: Administration of SBT( 2.6mg/kg and 5.2mg/kg) exerts a protective effect against damaging effects of ACM ( 750 mg/kg) on hepatocytes in albino rats. This hepatoprotective role becomes greater with increasing dose of SBT.
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